Jui-Heng Lin^{1}, Huei-Chun Hsiao^{1}, Shao-Chieh Lin^{1}, Yi-Jui Liu^{2}, Ruey-Hwang Chou^{3}, Ke-Sin Yan^{3}, Tan-Wei Liao^{3}, Chao-Chun Lin^{4}, Chia-Wei Lin^{4}, and Wu-Chung Shen^{4}

A non-Gaussian kurtosis model has been shown to take into account tissue heterogeneity and two relative imaging biomarkers namely, the kurtosis coefficient and the corrected diffusion coefficient can be quantified. In this study, 3D cell culture with hydrogels ECM was used to investigate whether DKI may provide information on these microenvironmental parameters and the microenvironment-associated metastatic propensity of tumors. Our results demonstrated DKI-MRI may provide the potential biomarkers on the change of microenvironment in the application of 3D cell culture experiment.

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Figure 1. A 3D cell culture with 6.4x10^{5}
, 3.2x10^{6} and 6.4x10^{6} cells/ml in 9-well dish from right
to left.

Figure 2. The DWI b0 image of 3D cell culture corresponding with Fig. 1 and the
selected ROI on one well.

Figure 3. The microscopic photos in the
wells of (A) 6.4x10^{5} ,(B) 3.2x10^{6} , and (C) 6.4x10^{6}
cells/ml.

Figure 4. (a) ADC, D and (b) Dk of different cell density measured mean and SD (x1: 6.4x10^{5}
,x2: 3.2x10^{6} , and x3: 6.4x10^{6} cells/ml).