Beyond B-cells: Neutropenia in the Setting of B-cell Depleting Therapies for Demyelinating Diseases
Clare Lambert1, Asli Buyukkurt1, Martin O'Donnell1, Angeliki Filippatou1, Elias Sotirchos1, Pavan Bhargava1, Peter Calabresi1, Michael Kornberg1, Carlos Pardo-Villamizar1, John Probasco1, Anne Yacoub1, Lisa Fox1, Moira Baynes1, Willard Wilson Will III1, Janel Haughton1, Nicole Pellegrini1, Sarah Snoops1, Scott Newsome1, Ellen Mowry1, Bardia Nourbakhsh1, Shiv Saidha1
1Neuroimmunology, Johns Hopkins University
Objective:

To investigate neutropenia in people with demyelinating diseases undergoing B-cell depleting therapies. 

Background:

Transient asymptomatic neutropenia is a rare complication of monoclonal antibody medications. There are limited studies on this complication in real-world populations. 

Design/Methods:

Patients seen by a neuroimmunologist at our tertiary referral center from 2008-2025 who had an international classification of disease (ICD) code for neutropenia were screened. Cases with confirmed concomitant anti-CD20 use were retained. The period prevalence of anti-CD20 use was estimated from ICD codes for demyelinating diseases and any anti-CD20 medication within their medication list. 

Results:

Among all 2912 patients treated with anti-CD20s at our center from 2008-2025, neutropenia was observed in 35 individuals (1.2%). Episodes of neutropenia occurred with ocrelizumab (n=24), rituximab (n=8), ofatumumab (n=9) and ublituximab (n=1). The average age at neutropenia onset was 41.1±10.7 years and 91% had multiple sclerosis. 12 patients presented with fever, sepsis and/or mucous membrane sores and 11 required hospitalization. 13 cases had absolute neutrophil counts <500 cell/µL; 6 required treatment with granulocyte colony stimulating factor. The neutropenia was deemed a drug related adverse event in 7, infectious sequelae in 2, “benign”/genetic or cyclic in 18, autoimmune in 2 and of unclear etiology in 6. Disease modifying therapy was changed in 12 patients; 4 stopped all therapies, 3 were switched to a fumarate, 2 moved to extended interval dosing, and 3 switched to other B cell depleting therapies. However, within this latter group, 2 subsequently developed recurrent neutropenia. 

Conclusions:

In our experience, patients who had asymptomatic cyclic or presumed “benign” neutropenia tolerated B-cell depletion well, whereas neutropenia as an adverse drug effect, associated with sepsis/fever, prompted medication switches. Neutropenia did not always spontaneously reverse and in some people, neutropenia appeared to be a class effect of B-cell depleting therapies, possibly reflecting presence of an inherent susceptibility. 

10.1212/WNL.0000000000217004
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